Blood lactate level in Wistar rats after four and twelve week intermittent aerobic training.

Background: Aerobic training can be done not only continuously, but also intermittently. Intermittent aerobic training aimed to get blood lactate level lower than continuous aerobic training. Blood lactate concentration in one of the various factors that determine training performance. However, until recently, little studies about intermittent aerobic training and blood lactate levels have been done. Therefore, this study aimed to measure blood lactate levels in Wistar rats after 4 and 12 weeks of intermittent aerobic training. Methods: 16 Wistar rats were divided into two groups, control and aerobic group. Every group was divided into two subgroups, 4-week and 12-week subgroup. Aerobic group performed training using T-6000 treadmill with a speed of 20 m/minute for 20 minutes, with resting period for 90 seconds every 5 minute. Measurements of lactate level was done with L-lactate (PAP) Randox kit (LC2389). Results: Blood lactate level in the 4-week aerobic group was 2.11 mmol/L, while that of the 4-week control group was 1.82 mmol/L (p > 0.05). Meanwhile, lactate level in 12-week aerobic group was 1.71 mmol/L (p < 0.05), and significantly lower than in 12-week control group, which was 3.03 mmol/L. Conclusion: This study showed that lactate level after 12-week intermittent aerobic training was the lowest compared to 4-week intermittent aerobic and 12-week control group.

Effect of long-term physical training and detraining on myocyte structure and connective tissue of Wistar rat’s ventricle: preliminary experiment in rats.

Background: This study was aimed to evaluate the structural changes of the ventricular myocardium in a physiological hypertrophic heart model due to long term aerobic and anaerobic physical training and detraining. Methods: In-vivo experimental study on Wistar rats (8 weeks old), weighing 150-250 grams who were divided into 3 large groups: control group, aerobic exercise group and anaerobic exercise group. Aerobic and anaerobic training were conducted for 4 and 12 weeks. At the end of 4 and 12 weeks of exercising, half of each exercising group was sacrificed to study the morphological and histopathological changes in myocardial structure. The remaining of the groups were given a period of 4 weeks of detraining and sacrificed at the end of the 8th and 16th week. Results: Significant differences in heart weight and left ventricular wall thickness was found in the 4 weeks of aerobic and anaerobic group compared to the control group (751.0 ± 36.5 gr and 791.1 ± 15.8 gr vs 588 ± 19.4 gr ), (3.34 ± 0.12 mm and 3.19 ± 0.1 mm vs 2.80 ± 0.07 mm). An increase in heart mass weight was observed in both 12 weeks aerobic and anaerobic training group compared to the control group (1030.8 ± 82.4 gr and 1140.4 + 0.24 gr vs 871.6 ± 62.0 gr). Heart volume of the 12 weeks aerobic-anaerobic groups showed a significant increase (3.58 ± 0.31 mm and 4.04 ± 0.30 mm) compared to the control group (2.82 ± 0.14 mm). The length of cardiomyocyte was in log 10 to normalize the data. There was a significant increase in the length of the cardiac muscle cells of the 4 weeks aerobic and anaerobic group (1.09± 0.08 μm and 1.00± 0.12 μm) compared with the control group (0.73± 0.1 μm). Width of heart muscle cells in the 4 weeks aerobic-anaerobic group showed a significant increase when compared to the control group (5.38± 1.3 μm and 5.5± 2.11 μm) vs (2.74± 0.53 μm). Significant reduction in the length of cardiac muscle cells in the detrained 4 weeks aerobic group (0.94± 0.08 μm) was found when compared to the treatment group (1.09± 0.08 μm). Significant differences were found between the length of cardiac muscle cells in the 12 weeks aerobic-anaerobic groups (1.3± 0.04 μm and 1.2± 0.07 μm) compared to the control group (0.95± 0.69 μm). Significant width increments of heart muscle cells was found in the 12 weeks aerobic-anaerobic groups (7.3± 1.01 μm and 6.44± 0.08 μm) compared to the control group (4.52 ± 0.91 μm). Conclusion: Long term aerobic and anaerobic training causes an increase in both wall thickness and diameter of the left ventricular cavity, as well as slight fibrosis. The increase in wall thickness, diameter, and fibrosis diminish during detraining period.